ABSTRACT
Objective To establish the background information of physiological parameters for germ?free ( GF ) Taihu piglets. Methods In this study we selected 25 days old GF Taihu piglets and 4 conventional ( CV) littermates, the male and female ratio was 1∶3, to measure the normal clinical values of hematology and serum biochemistry, immunoglobu?lin concentration and main organ coefficients. The analysis of relative growths of main organ weight to body weight was con?ducted in the Taihu GF and CV pigs by allometric scaling model. Results (1) Twelve hematological parameters and 8 blood biochemical parameters in the GF piglets were significantly lower than those in CV pigs (P<0?05). (2) The aver?age body weight, IgM concentration of GF pigs and CV pigs had significant difference ( P <0?05 ) , and no mesenteric lymph nodes were found in the GF pigs. (3) The gut weight had the largest allometric association with body weight in the GF pigs, while spleen weight has the largest allometric association with body weight in the CV pigs. Both the weight of heart and stomach in CV and GF pigs had a negative allometric association with body weight (allometric coefficient b<1), respectively. Conclusions Different microbe control grades affect the body weight, hematology and serum biochemistry, expression of immunoglobulin and development of main organs in laboratory pigs.
ABSTRACT
Germ-free ( GF) pigs are a special and adaptable experimental animal model for biomedical studies and animal productions, which are negative for bacteria, viruses, yeast and fungi tested by current microbiological examination. GF pigs were initially used in cleanse of epidemic diseases in animal production and in a bid to study the relationship be-tween animal disease and intestinal flora.Because of the similarities to humans in anatomy, physiology and hematology, and the clear microbiological background, GF pigs have been playing an important role in detecting the relationship between intestinal flora with growth and the development of diseases in medical biology, and also providing a special medical animal model for intestinal flora targeted prevention, diagnosis and treatment for update technology research in the clinic.This pa-per reviews the characteristics, advancements and research tendency of GF piglets.
ABSTRACT
Objective Sum up the therapentic action of external fixator in limb compound fiacture and comminuted fracture to improve the cure rate. Methods Select the external fixator in renson. Small extremitas superior and inferior. Results 264 cases patient, 224 cases follow up, heal rate over 84.8%. Conclusion Advantage for parenchyma repair and fracture heal.